HMP: The Bugs Selected for Sequencingbio1
October 28th, 2009
The collection of human and microbial cells in our body produce a vast milieu of proteins through their collective genomes. These proteins function as 3-D structures that are either neutral, beneficial or harmful to us. As we look into their functioning in our body, how they combine to make the many necessary and beneficial pathways and structures, we will also see ways in which certain pathogenic proteins hamper, inhibit or destroy pathways and structures, causing our body to malfunction — effecting our long term survival—bringing the de-evolutionary hypothesis into reality.
Let’s look at what bugs are presently being sequenced or are targeted to be sequences by the Human Microbiome Project (HMP) as members of the selected group of microbes to be called the reference genomes. They were chosen by a broad consensus of scientists worldwide because they are thought to be the most likely residents of the microbiomes of the gastrointestinal tract, skin, nasal, oral, urogenital and airways. Here are the questions I will address in the coming emails:
1. What are the bugs that have been selected for full genomic sequencing—to be members of the Reference Genome?
2. What is the evidence of their symbiotic relationships with our human cells, tissues and systems—specifically what pathways, receptors, enzymes and structures do they help facilitate?
3. What is the evidence demonstrating that certain pathogens in our microbiome weaken our health and vitality? And how do they lead us down the proverbial de-evolutionary path?
Lets take a look at how the HMP answers these questions.
I have discussed the issue of taxonomy many times with you individually, in classroom lectures, and in this newsletter. So far, the commercial probiotic industry has not gone through the universal scientific process of nomenclature, as all other biological sciences have done since the age of Aristotle. Almost all companies have named their species, and most certainly their subspecies, with propriety designations, to protect their “intellectual property”. This type of behavior thwarts of whole scientific process of discovery. We don’t know what bugs we are actually buying as consumers, since these organisms are not recognized with the universal scientific name, such as ATCC and other international repositories provide. As of this date, claims about organisms, are just that—in-house claims. The commercial industry needs to join the efforts of HMP and allow their organisms to go through the universal typing procedure—and get universal designations for their organisms. As you know, I have much passion for this field, and strongly believe that science needs to come first, before any commercial venture occurs.
The HMP has developed a detailed set of guidelines for inclusion of a strain in the reference genome group. They also welcome suggestions for inclusion of strains yet under consideration from groups outside their own.
The Genome Sequencing Centers for the HMP are located at The Broad Institute (of MIT and Harvard, Washington University, Baylor College of Medicine- Human Genome Sequencing Center (BCM-HGSC) and J. Craig Venter Institute.
The percent breakdown of numbers of bacterial species to be sequenced and their respective ecological niches are: GI tract- 27% (count-307), Oral- 23% (count-269), Skin- 19% (count-220), Urogenital tract-18% (210), Airways- 12% (count-138), Blood-1% (count-7), Heart- 0% (count- 1) and Eye- 0% (count-1).
Next week we will zero in on some of the GI tract selected species and the specifics of their genomic ability enabling their proteomic contribution to the overall functioning of our body. Exciting isn’t it!
In our two previous newsletters we have been looking at the two grouping of our synbiotic formulas—the American collection of bugs (in Oct. 7th email/newsletter) and their therapeutic food mix, and the last week the Bulgarian collection. All of our probiotics have gone through the toxonomy process.
As you can see in the picture below we have seven products left to discuss—let’s go over the four on the right. I classify these as our Oxidative Stress Reducing therapeutic foods—foods that clearly support the body’s Antioxidant Defense System.
On the far right we have Cruciferous Sprouts, and to their left we have wild blueberries —endogenous antioxidant support and exogenous antioxidant support respectively.
Cruciferous Sprouts Complex (powder) and the Cruciferous Sprouts Complex (capsules) have a slightly different profile of cruciferous sprouts (click on the links to see). The capsuled product was made for those individuals who don’t like the taste of cruciferous.
How to use and remember: Take one teaspoon or four capsules daily to enhance P2P production in every cell in the body. The glucosynolate family of molecules within this product trigger not just liver cells but all cells DNA to transcribe the production of the phase II enzymes (P2Ps) and therefore you are enhancing the body’s own ability to produce certain proteins that counteract inflammation, oxidation and that are crucial for detoxification. This ability we term endogenous Antioxidant Defense System support.
Wild Blueberry Daily and Wild Blueberry Extract were created to bring in the antioxidant, anti-inflammatory and neuro-regenerative power of the blueberry into our bodies to reduce oxidative stress. Clinical trails have clearly demonstrated the blueberry’s ability to enhance brain function—protecting it from oxidation. The same can be said for its protective benefits within our GI tract. The key portion of the blueberry providing these benefits are contain within its polyphenols—found within their purple pigment; and, is the reason we extracted the pigment to create the Wild Blueberry Extract. Additionally, we selected the Nova Scotia Wild Blueberry because it was rated by our USDA as the number one berry in North America for its oxygen radical absorbent capacity (ORAC).
How to use and remember: It takes us ¾ of a cup of blueberry to fill one capsule of the Daily. It’s ORAC score per capsule is 2000. It takes 1 and ¼ cup so to fill one capsule of the Wild Blueberry Extract whose ORAC score is 4000 per capsule. Use the Daily for prevention and the Extract for correction.